LEPL L. Characterization of the Sakvarelidze strain Repository of the National Center for Disease Control and Public Health (DCSC) using new generation sequencing (BAA)



A) Objective: The DCJE should also investigate strains stored in archives of particularly dangerous pathogens and obtain high-resolution genetic information using next-generation sequencing equipment and appropriate software. B) Ways to implement the project:
1. DCJJ will also select 100 archived and newly isolated strains from 4 endemic species (Y. pestis, B. anthracis, Brucella spp. and F. tularensis) distributed in Georgia, and their initial sequencing will be performed using the new generation of Illumina MiSeq technology.
2. The genomes will be compared with a worldwide database and their polygenetic analysis. SNP methodologies will be provided for further research.
3. Up to 20 selected samples will be analyzed in detail, and their genomes will be re-sequenced using Sanger or PacBio technologies.
    C) Expected results. The resources of the DCJEC Genome Laboratory will be strengthened. The main employee of the project, Los Alamos National Laboratory (LANL), will be constantly informed about the latest technologies of the DCJEC genomic information Laboratory.
New genome assembly and analysis equipment will be provided by LANL. DCJEC staff and students participating in the project will learn this technique and use it to process the data obtained during the project.
The data obtained may contribute to the detection of new cases of biodiversity and bioterrorism in the future.



Project implementation dates: 01/11/15-31/10/18


Budget: $518,409


The person responsible for the implementation of the project: Gvantsa Chanturia